Isolation, Purification, Identification and Pathogenicity of Sclerotinia sclerotiorum (Lib.) de Bary Caused by Sclerotinia Rot of Chickpea

Sclerotinia sclerotiorum (Lib.) de Barry is a soil habitat plant pathogen, able of infecting more than five hundred host plant spp., all over the world and doing a crucial role in reduce the yield of economically important crops. Sclerotinia rot also known as stem rot, incited by S. sclerotiorum is a certical disease of chickpea. We done an experiment in which, a number of fungal isolation, purification, identification and pathogenicity from the infected plant materals. The plants appear typical symptoms like drooping of petioles and leaflets and in last stage scattered sclerotial bodies. The infected isolates observed on the affected tissues after re-inoculation and generate mycelial growth and sclerotia both plants and culture plates. On re-isolation it was base that the fungus was identical to the authentic isolate. It was found that the infection was much supreme in inoculated plants as compare to control.